proteome profiler human chemokine array kit (R&D Systems)

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R&D Systems proteome profiler human chemokine array kit

BNP treatment increased moLCs migration toward CCL21 and reduced <t>chemokine</t> production in activated moLCs. Monocytes were cultured in the presence of GMCSF, TNF-α, and TGF-β for 5 days supplemented with IL-4 for the first 48 hrs to differentiate moLCs, and treated with 10 nM BNP throughout the entire differentiation process. On day 4, moLCs were activated with CL075 and poly(I:C) and a combination of both for 24 hrs. (A) In Transwell migration assay, 1*10 6 cells were applied in the upper well of the Transwell plate, and in the bottom of the plate, it contains RPMI supplemented with CCL19 and CCL21. Migration of the cells was measured with flow cytometry. Data are presented as individual values with mean ± SEM. Statistical significance was determined by Two-way ANOVA followed by Tukey’s multiple comparison test. Normality of residuals was confirmed by QQ plot analysis. n=8 biological replicates per group (B) A chemokine array was performed using supernatants from BNP treated and TLR activated cells. Symbols with different colors represent individual donors. Data is presented as Mean ± SEM. One-way ANOVA with Tukey’s multiple comparison test was used for statistical analysis. n=2 biological replicates per group *P<0.05, **P<0.01, *** P<0.001, **** P< 0.0001. BNP, B-type natriuretic peptide; CL075; TLR7/8 agonist, thiazoquinoline compound; CTRL, Control; moLC, monocyte-derived Langerhans cells; Poly(I:C), TLR3 agonist, polyinosinic:polycytidylic acid.

Proteome Profiler Human Chemokine Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteome+profiler+human+chemokine+array+kit/Proteome+Profiler+Human+Chemokine+Array+Kit/pmc13043391-230-0-6
Average 95 stars, based on 73 article reviews

proteome profiler human chemokine array kit - by Bioz Stars, 2026-10

95/100 stars

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1) Product Images from "B-type natriuretic peptide attenuates TLR-induced cytokine and chemokine secretion in monocyte-derived Langerhans cells"

Article Title: B-type natriuretic peptide attenuates TLR-induced cytokine and chemokine secretion in monocyte-derived Langerhans cells

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2026.1791284

BNP treatment increased moLCs migration toward CCL21 and reduced chemokine production in activated moLCs. Monocytes were cultured in the presence of GMCSF, TNF-α, and TGF-β for 5 days supplemented with IL-4 for the first 48 hrs to differentiate moLCs, and treated with 10 nM BNP throughout the entire differentiation process. On day 4, moLCs were activated with CL075 and poly(I:C) and a combination of both for 24 hrs. (A) In Transwell migration assay, 1*10 6 cells were applied in the upper well of the Transwell plate, and in the bottom of the plate, it contains RPMI supplemented with CCL19 and CCL21. Migration of the cells was measured with flow cytometry. Data are presented as individual values with mean ± SEM. Statistical significance was determined by Two-way ANOVA followed by Tukey’s multiple comparison test. Normality of residuals was confirmed by QQ plot analysis. n=8 biological replicates per group (B) A chemokine array was performed using supernatants from BNP treated and TLR activated cells. Symbols with different colors represent individual donors. Data is presented as Mean ± SEM. One-way ANOVA with Tukey’s multiple comparison test was used for statistical analysis. n=2 biological replicates per group *P<0.05, **P<0.01, *** P<0.001, **** P< 0.0001. BNP, B-type natriuretic peptide; CL075; TLR7/8 agonist, thiazoquinoline compound; CTRL, Control; moLC, monocyte-derived Langerhans cells; Poly(I:C), TLR3 agonist, polyinosinic:polycytidylic acid.

Figure Legend Snippet: BNP treatment increased moLCs migration toward CCL21 and reduced chemokine production in activated moLCs. Monocytes were cultured in the presence of GMCSF, TNF-α, and TGF-β for 5 days supplemented with IL-4 for the first 48 hrs to differentiate moLCs, and treated with 10 nM BNP throughout the entire differentiation process. On day 4, moLCs were activated with CL075 and poly(I:C) and a combination of both for 24 hrs. (A) In Transwell migration assay, 1*10 6 cells were applied in the upper well of the Transwell plate, and in the bottom of the plate, it contains RPMI supplemented with CCL19 and CCL21. Migration of the cells was measured with flow cytometry. Data are presented as individual values with mean ± SEM. Statistical significance was determined by Two-way ANOVA followed by Tukey’s multiple comparison test. Normality of residuals was confirmed by QQ plot analysis. n=8 biological replicates per group (B) A chemokine array was performed using supernatants from BNP treated and TLR activated cells. Symbols with different colors represent individual donors. Data is presented as Mean ± SEM. One-way ANOVA with Tukey’s multiple comparison test was used for statistical analysis. n=2 biological replicates per group *P<0.05, **P<0.01, *** P<0.001, **** P< 0.0001. BNP, B-type natriuretic peptide; CL075; TLR7/8 agonist, thiazoquinoline compound; CTRL, Control; moLC, monocyte-derived Langerhans cells; Poly(I:C), TLR3 agonist, polyinosinic:polycytidylic acid.

Techniques Used: Migration, Cell Culture, Transwell Migration Assay, Flow Cytometry, Comparison, Control, Derivative Assay

Expressing:

Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection
Article Snippet: .. SF specimens from two AL and three PJI patients were analyzed using a Proteome Profiler Human Chemokine Array Kit (ARY017; R&D Systems, USA), which simultaneously measures the expression levels of 31 chemokines. .. Spot intensities on the protein arrays were quantified using ImageJ software (National Institutes of Health, USA).

Article Title: Cytokine and Chemokine-Associated Signatures Underlying Dermal Invasion and Skin Metastasis in Melanoma.
Article Snippet: The protein concentration was determined using the PierceTM Coomassie Bradford Protein Assay Kit (Thermo Fisher Scientific Inc.). .. The Proteome Profiler Human Chemokine Array Kit and the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, Inc., Minneapolis, MN, USA) were used to analyze protein expression according to the manufacturer’s protocol. ..

Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection.
Article Snippet: .. Protein array and enzyme-linked immunosorbent assay SF specimens from two AL and three PJI patients were analyzed using a Proteome Profiler Human Chemokine Array Kit (ARY017; R&D Systems, USA), which simultaneously measures the expression levels of 31 chemokines. .. Spot intensities on the protein arrays were quantified using ImageJ software (National Institutes of Health, USA).

Article Title: A CD40-Targeted IL-21 Fusokine Enables Rapid Generation of Human IL-10⁺Granzyme B⁺ Regulatory B Cells
Article Snippet: .. Chemokine expression was assessed using the Proteome Profiler Human Chemokine Array Kit (R&D Systems, ARY017) according to the manufacturer’s instructions. ..

Article Title: Cytokine and Chemokine-Associated Signatures Underlying Dermal Invasion and Skin Metastasis in Melanoma
Article Snippet: The protein concentration was determined using the PierceTM Coomassie Bradford Protein Assay Kit (Thermo Fisher Scientific Inc.). .. The Proteome Profiler Human Chemokine Array Kit and the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, Inc., Minneapolis, MN, USA) were used to analyze protein expression according to the manufacturer’s protocol. ..

Protein Array:

Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection.
Article Snippet: .. Protein array and enzyme-linked immunosorbent assay SF specimens from two AL and three PJI patients were analyzed using a Proteome Profiler Human Chemokine Array Kit (ARY017; R&D Systems, USA), which simultaneously measures the expression levels of 31 chemokines. .. Spot intensities on the protein arrays were quantified using ImageJ software (National Institutes of Health, USA).

Produced:

Article Title: Midkine, a novel MCP-1 activator mediated PM2.5-aggravated experimental pulmonary fibrosis.
Article Snippet: .. To detect chemokines produced by cells, cell supernatants were collected after treatment and detected by using the Proteome Profiler Human Chemokine Array Kit (cat. no. ARY017, R&D Systems, Inc.) at Shanghai Universal Biotech Co., Ltd. ..

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